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Image Search Results
Journal: Blood Transfusion
Article Title: The tandem CD33-CLL1 CAR-T as an approach to treat acute myeloid leukemia
doi: 10.2450/BloodTransfus.786
Figure Lengend Snippet: ( A ) Expression of CD33 and CLL1 on the surface of human leukemia cell lines (HL-60, MV4–11, THP-1, U937, MOLM-13) was detected by flow cytometry. The gray line represents the isotype control. ( B ) Expression of CD33 and CLL1 in peripheral blood mononuclear cells (PBMCs, No.=22) and bone marrow (BM, No.=12) of Primary AML patients (down panel). A representative flow plot showed the expression of CD33 and CLL1 in tumor cells from two AML patients (up panel). ( C ) The structure of CD33-CAR-T (targeting CD33), CLL1-CAR-T (targeting CLL1), and CD33-CLL1- CAR-T (targeting both CD33 and CLL1). ( D ) The expression of CD45RO and CD62L on T cells before (un T)and after transduction (CAR-T) were detected using flow cytometry. CAR-T: chimeric antigen receptor T-cells; unT: untransfected T-cells.
Article Snippet:
Techniques: Expressing, Flow Cytometry, Control, Transduction
Journal: Blood Transfusion
Article Title: The tandem CD33-CLL1 CAR-T as an approach to treat acute myeloid leukemia
doi: 10.2450/BloodTransfus.786
Figure Lengend Snippet: ( A ) Killing percentages of HL60, U937, THP-1, and MOLM-13 cells by unT, CD33-CLL1-CAR-T, CLL1-CAR-T, and CD33-CAR-T cells after 18 h co-culture in vitro . E: T ratios mean the ratios of the absolute number of CAR-T cells to target cells. ( B ) Cytotoxic effects of CD33-CAR-T, CLL1-CAR-T, and CD33-CLL1-CAR-T on 3 primary AML blasts (based on the expression of CD33 and CLL1) after 18 h co-culture in vitro (E: T=1:4). The data were represented as means ± SD and analyzed by One-way ANOVA and two-tailed unpaired Student’s t-test. *p<0.05; **p<0.01; ***p<0.001, ns, no significance.
Article Snippet:
Techniques: Co-Culture Assay, In Vitro, Expressing, Two Tailed Test